Journal: Cell systems

Article Title: Lattice Light-Sheet Microscopy Multi-Dimensional Analyses (LaMDA) of T-Cell Receptor Dynamics Predict T-Cell Signaling States

doi: 10.1016/j.cels.2020.04.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488 anti-mouse TCR β chain Antibody BioLegend Cat# 109215; RRID: AB 493345 Purified anti-mouse CD4 Antibody BioLegend Cat#: 100401; RRID: AB 312686 Purified anti-mouse CD28 Antibody BioLegend Cat#: 102101; RRID: AB 312866 Purified anti-mouse anti-CD3e University of Chicago Monoclonal Antibody Facility Clone 145–2C11 Chemicals, Peptides, and Recombinant Proteins RBC Lysis Buffer eBioscience Cat#: 00-4300-54 Moth Cytochrome C (MCC), sequence ANERADLIAYLKQATK Elimbio N/A K5, sequence ANERADLIAYFKAATKF Elimbio N/A 102S, sequence ANERADLIAYLKQASK Elimbio N/A Ficoll-Paque Plus GE Healthcare Cat#: 17-1440-02 Recombinant mouse IL-2 Sigma-Aldrich Cat#: I0523 Poly-L-Lysine Phenix Research Products Cat#: P8920–100ML PP2 (4-Amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine) Millipore Sigma Cat#: P0042 Retronectin Clontech Cat#: T100A Recombinant human IL-2 Peprotech Cat#: AF-200–02 Critical Commercial Assays MojoSort Mouse CD4 T Cell Isolation Kit BioLegend Cat#: 480033 Thermo Scientific Pierce Fab Micro Preparation Kits Thermo Fisher Scientific Cat#: 44685 Experimental Models: Cell Lines Mouse: CH27-mCherry Laboratory of Dr. Enfu Hui N/A Experimental Models: Organisms/Strains Mouse: B10.A-Rag2−/− H2-T18a Tg (Tcra5CC7,Tcrb5CC7) NIH N/A Recombinant DNA MIG-CD3ζ-GFP Laboratory of Dr. Hans Schrieber N/A Software and Algorithms Slidebook 3i https://www.intelligent-imaging.com/slidebook Imaris Oxford Instruments https://imaris.oxinst.com/ LLSpy LLSpy was used under license from Howard Hughes Medical Institute, Janelia Research Campus.

Techniques: Purification, Recombinant, Red Blood Cell Lysis, Sequencing, Cell Isolation, Software, Microscopy